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KMID : 0914820010010040202
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Journal of the Korean Gastric Cancer Association
2001 Volume.1 No. 4 p.202 ~ p.209
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E-cadherin and beta-catenin Expression and Mutation in Gastric Carcinomas
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Kim Kwang-Il
Park Sung-Hye
Han Sun-Ae
Chae Yang-Seok
Kim In-Sun
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Abstract
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Purpose: When cancer cels invade the stroma, they should be dissociated from the adjacent cells at first. E-cadherin and beta-catenin constitute an important protein complex associated with cellular adhesion, development, and differentiation, especially in epithelial cells. The role of E-cadherin and beta-catenin in gastric carcinogenesis were studied.
Materials and Methods: The expression of E-cadherin and beta-catenin in gastric adenocarcinomas by using immunohistochemical staining and the mutation by using polymerase chain reaction- single stranded conformation polymorphism (PCR-SSCP) and sequencing were performed in 40 adenocarcinomas and 5 dysplasia of stomach. Thirteen cases, which had lymph node metastasis, were also included for immunohistochemical staining.
Results: Inappropriate cytoplasmic and/or nuclear expression of a E-cadherin-beta-catenin complex was more frequent in poorly differentiated, diffuse type signet ring cell carcinomas than in well-differentiated, intestinal type adenocarcinomas (P<0.05). However, the expression was not related with clinical stage or lymph node metastasis. Mutation of E-cadherin was detected in 4 cases by using PCR-SSCP, whereas mutation of beta-catenin was detected in 2 cases.
Conclusion: E-cadherin and beta-catenin seem to be important in gastric carcinogenesis, especially in poorly differentiated diffuse type.
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KEYWORD
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Gastric carcinoma, E-cadherin, β-catenin, Immunohistochemistry
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